Editorial Commentary:Is Real‐Time Polymerase Chain Reaction Ready for Real Use in Detecting Candidemia?

Is real-time polymerase chain reaction ready for real use in detecting candidemia?

Received 27 November 2007; accepted 28 November 2007; electronically published 7 February 2008. Reprints or correspondence: Dr. John Bennett, Clinical Mycology Section, NIAID, Clinical Center room 11N234, National Institutes of Health, Bethesda, MD 20892 ([email protected]). Clinical Infectious Diseases 2008; 46:897–8 This article is in the public domain, and no copyright is claimed. 1058-...

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*

Real-time polymerase chain reaction.

Real-time PCR is the state-of-the-art technique to quantify nucleic acids for mutation detection, genotyping and chimerism analysis. Since its development in the 1990s, many different assay formats have been developed and the number of real-time PCR machines of different design is continuously increasing. This review provides a survey of the instruments and assay formats available and discusses...

Development of New Modified Simple Polymerase Chain Reaction and Real-time Polymerase Chain Reaction for the Identification of Iranian Brucella abortus Strains

Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified as biological threat agents. In this regard, the identification of Brucella can be a time-consuming and labor-intensive process posing a real risk of laboratory-acquired infection to the laboratory staff. This stud...

Detection of Nocardia Asteroides Complex in Clinical Isolates by Real-Time Polymerase Chain Reaction

Background and Aims: Nocardia asteroides complex is the most common cause of infectious diseases due to nocardiosis. Interspecies differentiation of Nocardia genera is essential for prognosis and timely proper treatment, as well as for epidemiological studies. Since each genus has its own antibiotic resistance, precise careful diagnosis is of prime importance. As compared to biochemical and phe...